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Zebrafish were kept in groups of seven to nine and fasted 24 hours before injection. They were anaesthetized with MS-222 (Sigma-Aldrich) and injected intraperitoneally using an insulin syringe with a 30-gauge needle (Bunzl Healthcare, 324826) with TPT hydrochloride (Sigma-Aldrich) or 30% DMSO in sterile Hank’s buffered solution (Gibco, 11530476). A daily dose of TPT hydrochloride (22.5 mg/kg) was injected for two consecutive days for a final concentration of 45 mg/kg in a total volume of 10 μl.
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