Quantification of intestinal lumen width

Young adult N2 worms were exposed to E. Coli OP50 lawn and P. aeruginosa lawn in the same bacterial lawn size setup as done in the aversion response assay and incubated at 25°C for 12 h. The worms were then randomly picked from inside or outside of the lawn; washed with M9 buffer containing 50 mM sodium azide, and mounted on 2% agar pads. The worms were visualized using Leica DMi8 inverted fluorescence microscope.