After cleaning the leaves with 10 mM calcium chloride solution25 followed by deionized water, FW of the leaves was measured immediately and DW was obtained following incubation at 105 °C for 15 min and drying to constant weight at 70 °C for 2 days23. Five replicates were performed per variety and treatment. The reduction rate was calculated as (FW under control condition—FW under saline condition)/FW under control condition × 100%. The same method was processed in calculating the reduction rate of DW. The relative water content is calculated as (FW-DW)/FW × 100%26.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.