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2 × 106 cells/ml tumor cells resuspended in 100 μl RPMI1640 (containing 1% FBS) were seeded into 8-μm pore polycarbonate filters, and complete medium (containing 10% FBS) was added into the bottom well. After 16 h culture, the non-migratory cells on the top of the filters were removed by cotton swab, and the migratory cells on the bottom of filters were dyed with 0.1% crystal violet for 10 min. The crystal violet could be eluted by 33% acetic acid, and the absorbance value of eluent was read out by Elisa (Biotec) at the wavelength of 570 nm.
Copyright and License information: ©2021 Wang, Ma, Wang, He, Chen, Duan, Li, Si, Chuang, Chen and Luo
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