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4.5. Gut Microbiota Analysis by 16S rRNA Gene Sequencing
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Fecal samples and cecal contents were used for gut microbiota analysis by sequencing the 16S rRNA genes. The DNA extraction, PCR amplification, sequencing, and data analysis were conducted according to our previous study [11].
Copyright and License information: ©2021 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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