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Cellular apoptosis was quantified by flow cytometry assay after annexin V–FITC and propidium iodide staining. According to instructions, cells were detached with 2.5% trypsin-EDTA and centrifuged at 1500 rpm for 5 min. The pellet was suspended in 300 µL of 1× binding buffer (FITC Annexin V Apoptosis Detection Kit I, BD Biosciences, USA). Flow cytometry and data analysis were conducted on a BD FACSCalibur.
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