2.5. Primers for Biofilm Genes Used in RT-qPCR

Table 1 shows the primers used for the RT-qPCR. Primers were synthetized by Metabion (Metabion GmbH, Planegg/Steinkirchen, Germany). The lyophilized primers were diluted to 100 µM following the manufacturer’s recommendations. Primers were then diluted to 10 µM stock solutions for further use. For the two S. epidermidis strains the primers were synthetized based on the references mentioned in Table 1. For the other strains the corresponding gene sequences (Table 2) were acquired from GeneBank and primers were designed by Primer-Blast (National Center for Biotechnology Information—NCBI, Bethesda MD, MD, USA) [22].

Forward and reverse primers for all strains used in this study.

Accession numbers for the genes and their corresponding strains used.