2.4. Gene Expression Analysis

For the RNA isolation we used the Qiagen RNeasy Mini Kit (RNeasy^® Mini Kit, Qiagen GmbH, Hilden, Germany). DNase treatment was performed with DNase I (DNase I RNase-free, 2000 U/mL, New England Biolabs, Frankfurt am Main, Germany). For the cDNA synthesis we used the iScript^TM reverse transcription Supermix for RT-qPCR (iScript^TM RT Supermix, Bio-Rad Laboratories, Feldkirchen, Germany) and the PicoReal 96 System (PikoReal 96 Realtime PCR System, Thermo Fisher Scientific, Waltham, MA, USA) for the thermo cycling. The real-time qPCR was performed with the iQ^TM SYBR Green Supermix (iQ^TM SYBR Green Supermix, Bio-Rad Laboratories, Feldkirchen, Germany) and the PicoReal 96 System (PikoReal 96 Realtime PCR System, Thermo Fisher Scientific, Waltham, MA, USA).