Morphological, physiological, and biochemical identification tests

Motility, Gram stain, spore formation were done for Morphological tests. Physiological tests for the effect of different pH values on growth, temperature, and NaCl tolerance as well as gelatin liquefaction was determined according to Bergey's Manual of Determinative Bacteriology⁷⁹. For testing the ability of isolates to grow under anaerobic conditions, bacterial isolates were tested using AnaeroGen jar 2.5 liters⁸⁰.

Biochemical characterization tests were performed using diagnostics ENC 8 kit and GN 24 kit identification system for Gram-positive and Gram-negative bacteria, respectively. As well DNAase test was performed by streaking each bacterial culture on the DNAase agar plate (HIMEDIA M482) and incubated at 28 ± 2 °C for 3 days, then the plate was flooded with 0.1% toluidine blue. A clearance zone around the colonies was recorded as a positive reaction⁸¹.