4.9. Neurotoxicity Assay

Neurotoxicity was induced by treating wild-type (N2) and transgenic (CF1038 and CB1370) worms with MPP⁺. L1 larvae were added to 96-well plates (15 worms/well) in a 40 μL solution containing E. coli OP50. Worms were then incubated with 50 μL of 0.75 mM MPP⁺ alone or in the presence of different concentrations of the tested sample for 48 h. After incubation, worm viability was visually inspected under a stereomicroscope. Live worms were counted every 12 h post treatment until no live worms remained. The results of the MPP⁺-treated groups were normalized and expressed as a percentage of the normal controls. The results were obtained from three independent experiments (80–130 worms/treatment in each experiment).