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4.3. Nitrogen Balance

GC Gabriela de Matuoka e Chiocchetti
LL Leisa Lopes-Aguiar
NM Natália Angelo da Silva Miyaguti
LV Lais Rosa Viana
CS Carla de Moraes Salgado
OO Ophelie Ocean Orvoën
DF Derly Florindo
RS Rogério Williams dos Santos
MG Maria Cristina Cintra Gomes-Marcondes
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From each animal kept in individual metabolic cages, the nitrogen balance refers to nitrogen incorporation by food intake (mg N2 daily) minus the nitrogen excretion through faecal material (mg N2 in the total faecal mass in 24 h) and urinary excretion (mg N2 in the total volume of urine in 24 h). The diary nitrogen content in the diet, faeces, and urine were measured by using a colorimetric micro-Kjeldahl method. The results are presented as a percentage of mg N2 in 24 h vs. the initial value [67].

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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