The recombinant humanised monoclonal HER2 antibody trastuzumab (a concentration of 15 µg/mL was selected as indicated elsewhere [24]) (Herceptin, Genentech, San Francisco, CA, United States) was supplied by the pharmacy of our hospital; BEZ235 (S1009), everolimus (S1120) and TAK-228 (S2811) were obtained from Selleckchem (Selleckchem Spain, Madrid, Spain).
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