Bilateral 4T1 and EMT6 tumor model

YH Yanjuan Huang
ZG Zilin Guan
XD Xiuling Dai
YS Yifeng Shen
QW Qin Wei
LR Lingling Ren
JJ Jingwen Jiang
ZX Zhanghong Xiao
YJ Yali Jiang
DL Di Liu
ZH Zeqian Huang
XX Xiaoyu Xu
YL Yong Luo
CZ Chunshun Zhao
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To establish a bilateral 4T1 and TMT6 tumor model, female Balb/c mice (18-20 g) were injected subcutaneously with 1 × 106 4T1 or EMT6 cells in the left flank (primary tumor), one week or 6 days after inoculation of 4T1 or EMT6 respectively, 1 × 105 4T1 cells or 5 × 105 EMT6 cells were injected into the medullary cavity of the right tibia through a 26-gauge needle under anesthesia (bone metastatic tumor).

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