Inhibitory effect on LPS-induced PGE2 production from RAW 264.7 cell

The inhibition effect of SHT and its plant components on the release of PGE₂ from RAW 264.7 cells was evaluated using a prostaglandin E₂ EIA kit (monoclonal) according to the manufacturer’s instructions. In brief, cell culture and sample treatment protocols were conducted similar to the study protocol of anti-TNF-α described above except for the concentration of LPS. In this assay, the production of PGE₂ from RAW264.7 was induced by LPS at a concentration of 5 μg/mL. The culture medium after sample treatment (0.1, 1, 10, 30 μg/mL) was collected and the amount of PGE₂ production was determined by the PGE₂ EIA kit, Indomethacin was used as a positive control. The inhibition (%) of PGE₂ production was calculated by the following equation, and IC₅₀ values (Table 2) were calculated from the Prism program.

Inhibitory effects (IC₅₀₎ on tumor necrosis factor-alpha and prostaglandin E2 production from RAW264.7 of the ethanolic extracts of Sahasthara and its plant components (n = 3).

where, OD (sample) stands for optical density of sample (treated in LPS-induced cells); OD (active), optical density of diluent (treated in LPS-induced cells); OD(inactive), optical density of diluent (treated in non-LPS-induced cells).