Endotoxemia model

Referring to the methods in the literature^9,10 and the previous experimental results in our group, the model of inducing endotoxin blood will be induced, and appropriate improvements will be made according to the actual situation. BALB/c mice were divided into four groups (n = 5): Control, lipopolysaccharide (LPS), LPS + C16, and LPS + SIN. According to the reports in the literature^11,12 and the previous work of the research group, the model of endotoxemia in vivo was induced by injecting 10 mg/kg LPS into the abdominal cavity of mice according to the body weight of mice. The state of mice was observed and the model was successful. In order to compare the anti-inflammatory effect of sinomenine before and after modification, high (5 mg/kg) and low (2.5 mg/kg) dose groups of sinomenine and sinomenine 4-hydroxypalmitate were designed to observe the vital signs and activities of mice within 24 h. There was no significant change in activity during the first 4 h after LPS treatment. The mice in the LPS group gradually died after about 12 h. The results showed that the effect of C16 was stronger than that of SIN, and the anti-inflammatory effect of high-dose group was stronger than that of low-dose group. All the mice were administered 5 mg/kg sinomenine (SIN) (Sigma-Aldrich, St. Louis, MO), 5 mg/kg C16, or PBS by i.p. injection before LPS challenge. And then endotoxemia was induced by i.p. injection of 0.2 mL of normal saline (NS) containing 10 mg/kg LPS (Sigma-Aldrich, St. Louis, MO). NC group was injected the same volume of PBS. Survival rate was monitored for 24 h.