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Axonal outgrowth was measured based on the NF-m staining. For tracking individual neurites, the semi-automated Fiji application plugin [39] NeuriteJ [40] was used. The mean length of neurites was measured and then averaged for ten of the most extended neurites per DRG, as described elsewhere [37]. Results are presented as a mean length of neurites calculated based on nine DRG per condition.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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