siRNA transfection

MH Michael Y. He
MH Michael M. Halford
RL Ruofei Liu
JR James P. Roy
ZG Zoe L. Grant
LC Leigh Coultas
NT Niko Thio
OG Omer Gilan
YC Yih-Chih Chan
MD Mark A. Dawson
MA Marc G. Achen
SS Steven A. Stacker
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Transfection of ECs for delivering siRNA was performed using a cationic lipid-mediated transfection reagent (Lipofectamine RNAiMAX transfection reagent, Life Technologies) according to the manufacturer’s instructions. siRNAs targeting specific genes were purchased as siGENOME siRNA SMARTpools from Dharmacon (each SMARTpool is an equimolar mix of four siRNAs targeting the same gene provided as a single reagent). The ON-TARGETplus Non-targeting Control Pool (referred to as OTP-NT; each pool contained an equimolar mix of four non-targeting siRNAs; Dharmacon) was used as a negative control. All siRNA pools were used at 20 nM unless specified otherwise.

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