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2.4. Synthesis of Pig T helper-GnRH with Alkyne Moiety (PT-GnRH)

MF Mohammad O. Faruck
PK Prashamsa Koirala
JY Jieru Yang
MD Michael J. D’Occhio
MS Mariusz Skwarczynski
IT Istvan Toth
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PT-GnRH (pentynoyl-KHKVRDEVMVHWFDD-EHWSYGLRPG) was synthesized analogously to PADRE-GnRH except pentynoic acid was coupled to the peptide N-terminus instead of the acetyl group. The crude compound was purified using preparative HPLC (C18 column). Molecular weight = 3203.63, ESI-MS [M + 2H]2+ m/z 1602.3 (calc. 1602.8), [M + 3H]3+ m/z 1068.5 (calc. 1068.8), [M + 4H]4+ m/z 801.5 (calc. 801.9). HPLC C18 column, tR 19.8 min, purity 99%, yield 43% (Supporting Information Figure S2).

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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