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Colony-forming efficiency (CFE) assays
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Cell suspension was plated at 300 cells/cm2 and medium was refreshed every 3 days. At 14 days in culture, the medium was removed, cells were fixed with 4% paraformaldehyde (PFA) for 15 min, washed twice with PBS, and stained with 1% crystal violet at room temperature for 30 min. The plates were rinsed with ample water, dried and photographed. Colonies were counted and CFE was calculated using a formula: (# colonies/#total cells plated) * 100.
Copyright and License information: ©2021 Wang et al ©2021 Majlinda LakoThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ©2021 Majlinda LakoThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ©2021 Majlinda LakoThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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