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Ratio Validation Using Experimentally Measured Abundance of Transcripts and Proteins

YB Yogendra Bhaskar
XS Xiaoquan Su
CX Chenggang Xu
JX Jian Xu
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The predicted ratios of the SRPS operons were validated using mRNA sequencing (mRNA-Seq) gene expression and mass spectrometry protein expression data. The gene expression data used from our cellulosome complex stoichiometry study (Xu et al., 2015) and two protein expression data were used to validate the predicted ratio: (i) the LC/MS data in this study (Supplementary Data 1B) and (ii) the LC/MS data from the cellulosome composition analysis of the Ccel study (Blouzard et al., 2010). The gene expressions for the other bacteria were downloaded from the Gene Expression Omnibus (GEO) (Edgar et al., 2002; Clough and Barrett, 2016) using the following dataset series: GSE22426, GSE18471, and GSE80786 (for Cthe, Cace, and Bsub, respectively). The raw datasets were downloaded and normalized using log(base 10). Pearson’s correlation was calculated between the predicted ratio and the actual operon ratio from the log of expression data.

Copyright and License information:  ©2021 Bhaskar, Su, Xu and Xu.
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