Determination of LPS-Induced Cytokine Release (IL-6 and IL-8).

Beth Malcomson; Hollie Wilson; Eleonora Veglia; Gayathri Thillaiyampalam; Ruth Barsden; Shauna Donegan; Amal El Banna; Joseph S. Elborn; Madeleine Ennis; Catriona Kelly; Shu-Dong Zhang; Bettina C. Schock

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Determination of LPS-Induced Cytokine Release (IL-6 and IL-8).

BM Beth Malcomson

HW Hollie Wilson

EV Eleonora Veglia

GT Gayathri Thillaiyampalam

RB Ruth Barsden

SD Shauna Donegan

AB Amal El Banna

JE Joseph S. Elborn

ME Madeleine Ennis

CK Catriona Kelly

SZ Shu-Dong Zhang

BS Bettina C. Schock

This method is extracted from research article: Proc Natl Acad Sci U S A, Jun 2016

Connectivity mapping (ssCMap) to predict A20-inducing drugs and their antiinflammatory action in cystic fibrosis

DOI: 10.1073/pnas.1520289113

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IL-6 and IL-8 in cell-free culture supernatants were measured by a commercially available ELISA (PeproTech EC Ltd.) according to the manufacturer’s instructions.

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