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2.3.2. Alkaline Hydrolysis and Neutralization
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Alkali hydrolysis was carried out using 25% NaOH. The pH was changed to 10.5 and then the extracts were sonicated at 45 ± 2 °C for 10 min. After hydrolysis, the sample was neutralized to pH 5.7 using 25% acetic acid. The neutralized extracts were filtered. The neutralized solution was prepared for further HPLC analysis.
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