Ribosome purification

TG Tolou Golkar
AB Angelia V. Bassenden
KM Krishnagopal Maiti
DA Dev P. Arya
TS T. Martin Schmeing
AB Albert M. Berghuis
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70S ribosomes were purified from HB8 T. thermophilus cells using the Selmer et al. purification protocol, with minor modifications to the final step. Here, zonal ultracentrifugation was replaced by three steps of 10–40% sucrose gradient preparation, ultracentrifugation, and fraction collection19.

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