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4.7. POX and PPO Activity Determination

AC Anka Cebulj
AV Andreja Vanzo
JH Joze Hladnik
DK Damijana Kastelec
UV Urska Vrhovsek
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For the enzyme activity, the frozen material from −80 °C of storage was used. We were following the protocol described in Zupan et al. [40] for the preparation of samples for the POX activity and Cebulj et al. [41] for the preparation of samples for the PPO activity. Measurements were made according to the Worthington manual (Worthington Biochemical Corporation, 1972). The enzyme activity was calculated in ΔA min−1.

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