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The DTNB protocol was used in the N-acetyltransferase assay (65). DTNB (Ellman’s Reagent, 22582) was purchased from Thermo Fisher Scientific. A positive control experiment was done using human Naa50 protein and its known substrates. The oligopeptides used in this study were custom made (Genscript): the peptides contained seven specific amino acids at their N termini (α-tubulin 84D: MRECISI/β-tubulin 56D: MLIGARP) followed by 17 amino acids identical to the adrenocorticotropic hormone peptide sequence (RWGRPVGRRRRPVRVYP), while the internal lysines were replaced with arginines to avoid interference caused by internal acetylation (Nε).
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