Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

LDH cytotoxicity assay

AM Amani Musa
EW Emanuel Wiman
RS Robert Selegård
DA Daniel Aili
TB Torbjörn Bengtsson
HK Hazem Khalaf
request Request a Protocol
ask Ask a question
Favorite

Cytotoxicity of S. aureus and PLNC8 αβ on HaCaT cells was determined by measuring the activity of extracellular lactate dehydrogenase (LDH) using the LDH cytotoxicity assay (Thermo Scientific ™ Pierce ™ LDH Cytotoxicity Assay Kit). The procedure was performed according to the manufacturer’s instructions. The method relies on the fact that the cytosolic enzyme LDH is released into the surrounding cell culture media if the cell membrane is damaged. Extracellular LDH undergoes an enzymatic reaction with the assay chemicals culminating in the formation of a red formazan compound, which is measured in a spectrophotometer at 490 nm.

Copyright and License information: The Author(s) ©2021
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A