To predict possible antigens and subunit vaccines, the identified target proteins were analyzed by the VaxiJenv2.0 server. We chose VaxiJen as it is a reliable and consistent tool for the prediction of protective antigens with a reported accuracy of 82% (Doytchinova and Flower 2007). This server was developed to classify antigens based on physicochemical properties. Protein sequences with antigenicity greater than 0.5 were considered antigens.
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