All statistical analyses were performed using GraphPad Prism v7 (GraphPad Software, San Diego, CA). Student's t tests were used to evaluate differences between the two treatment groups. Comparison of two or more genotype/treatment groups was made using one‐way or two‐way analysis of variance (ANOVA), followed by Fisher's LSD post hoc tests. The Log‐rank test and Gehan–Breslow–Wilcoxon test were used to compare survival curves between vehicle and Q134R‐treated mice. Significance for all statistical comparisons was set at p ≤ 0.05.
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