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2.8. Production and Infusion of Mouse IL-2-Activated NK Cells

RD Raynier Devillier
BC Boris Calmels
SG Sophie Guia
MT Mohammed Taha
CF Cyril Fauriat
BM Bechara Mfarrej
GV Geoffroy Venton
EV Eric Vivier
DO Daniel Olive
CC Christian Chabannon
DB Didier Blaise
SU Sophie Ugolini
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Splenocytes from C57BL/6 (CD45.1+) mice were used to isolate donor NK cells. NK cells were enriched by using the mouse NK Cell Isolation Kit II (Miltenyi Biotec) and activated in vitro for 5 days with 5.000 U/mL IL-2 (Chiron) before intravenous (IV) infusion into C57BL/6 (CD45.2+) recipient mice.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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