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Screening of DEMs and DEGs

XP Xiaotong Peng
ZZ Zhirong Zhang
YM Yanqun Mo
JL Junliang Liu
SW Shuo Wang
HL Huining Liu
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GEO2R (https://www.ncbi.nlm.nih.gov/geo/geo2r/) is an online analysis tool that allows for comparisons between choriocarcinoma cell samples and normal cell samples from normalized GEO datasets. The Benjamini and Hochberg false discovery rate (FDR) method was utilized to adjust the p-value to reduce false positivity. With the thresholds of adjusted p-value < 0.05 and |log2-fold change (FC)| > 1, the top 400 upregulated and downregulated given genes were selected as DEGs. Under the same conditions, DEMs were screened and analysed in overlapping sections of the GSE32346 and GSE130489 datasets through a Venn diagram to identify candidate DEMs.14 Heatmaps of DEMs and DEGs were visualized by TBtools, which is a toolkit that integrates various biological data handling tools.

Copyright and License information:  ©2021 Peng et al.
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