Cell lines

WJ Wulin Jiang
YY Yuchen Yang
AM Alison R. Mercer-Smith
AV Alain Valdivia
JB Juli R. Bago
AW Alex S. Woodell
AB Andrew A. Buckley
MM Michael H. Marand
LQ Li Qian
CA Carey K. Anders
SH Shawn D. Hingtgen
request Request a Protocol
ask Ask a question
Favorite

The hTERT-immortalized human fibroblast line NHF-1 was purchased from the University of North Carolina at Chapel Hill Tissue Culture Facility. The TNBC brain metastasis cell line MDA-MB-231-Br was obtained through material transfer agreement (MTA) (T. Yoneda). MDA-MB-231-Br-GFP-NLuc (Nano Luciferase) cells or MDA-MB-231-Br-mCherry-FLuc (Firefly Luciferase) cells were generated by transducing MDA-MB-231-Br cells with LV-GFP-NLuc or LV-mCherry-FLuc, respectively. Both cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v) fetal bovine serum (FBS; Millipore), 1% (v/v) penicillin-streptomycin (Gibco), and 0.2% (v/v) Plasmocin.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A