The hTERT-immortalized human fibroblast line NHF-1 was purchased from the University of North Carolina at Chapel Hill Tissue Culture Facility. The TNBC brain metastasis cell line MDA-MB-231-Br was obtained through material transfer agreement (MTA) (T. Yoneda). MDA-MB-231-Br-GFP-NLuc (Nano Luciferase) cells or MDA-MB-231-Br-mCherry-FLuc (Firefly Luciferase) cells were generated by transducing MDA-MB-231-Br cells with LV-GFP-NLuc or LV-mCherry-FLuc, respectively. Both cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v) fetal bovine serum (FBS; Millipore), 1% (v/v) penicillin-streptomycin (Gibco), and 0.2% (v/v) Plasmocin.
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