Effect of Different Application Methods

Effective strains were selected after in vitro and in vivo bioassay, to evaluate their effectiveness under field conditions against tobacco leaf mildew during curing at Seka Village, Reshui Township, Yunnan Province. Standard agronomic practices were followed in crop cultivation. Fresh tobacco leaves were soaked (300 leaves for each concentration) in a suspension of Y2 at 10⁵ CFU/mL or sprayed (1,000 leaves for each treatment) on the tobacco petiole top until the drop dripped. An untreated control was used as a negative test. In a completely randomized block design, treatments in each curing barn involved three replicates. Disease assessment was performed as described above after 7 days. Each experiment was carried out three times. Disease evaluation followed the same procedure as above.