4.10. Time-Killing Assay

A time-killing assay of the peptides against A. baumannii KCTC 2508 was performed. The bacteria were grown to mid-log phase in NB medium, with cell density of A. baumannii KCTC 2508 of 2 × 10⁵ CFU/mL. The peptides were used to treat the bacteria at concentrations of 1×, 2× and 4× MIC and incubated at 37 °C with shaking for 0–180 min. The reactive aliquots were plated on NB agar and incubated at 37 °C for 24 h, and surviving bacterial cells were counted. The assay was conducted in triplicate.