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Cell migration and invasion following TGFB treatment were assessed by Boyden Chamber assays as described in Supplementary Methods. Migration experiments were repeated following pretreatment with TGFBR1 inhibition. The percentage of cell invasion was calculated as follows: (mean of invading cells/condition divided by the mean of migrating cells/condition) × 100.
Copyright and License information: ©2021 The Author(s) 2021. Published by Oxford University Press, the Society for Neuro-Oncology and the European Association of Neuro-Oncology.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
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