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Caspase-1 activity was measured using colorimetric assay (BioVision, Mountain View, CA). The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage by caspase-1 from the labeled substrate YVAD-pNA. The pNA light emission can be quantified using a spectrophotometer at 405 nm. Comparison of the absorbance of a treated sample's pNA with that of an untreated control allows determination of the fold increase in caspase-1 activity.
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