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JSC-1 cells were treated with 1, 25(OH)2 D3, vehicle, or TPA for 48 h. Supernatants were harvested and added to confluent monolayers of uninfected 293 cells in a 24 well dish. Polybrene (8 µg/mL) was added to each well and the plate was spinoculated at 2500 rpm for 2 h at 26 °C as previously described40. Ninety-six hours post-infection, intracellular viral loaded was determined by real time PCR. Furthermore, infection was validated by checking the expression of LANA by western blot.
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