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Chlorophyll content was determined using the method described by Li et al. (2003). Arabidopsis leaves (0.2 g FW) were washed in distilled water and extracted with 80% acetone for 48 h in the dark. Absorbency at 663 and 645 were determined using a TU-1810 UV-spectrophotometer. Chlorophyll content was calculated as follows: Ca (mg/L) = 12.7A663 – 2.69A645; Cb (mg/L) = 22.9A645 – 4.68A663.
Copyright and License information: ©2017 Sui, Tian, Wang, Wang and Fan.
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