Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Young’s modulus measurement

YL Yunwoo Lee
SD Sreekantha Reddy Dugansani
SJ So Hee Jeon
SH Soon Hyoung Hwang
JK Jae-Hyun Kim
SP Sung Ha Park
JJ Jun-Ho Jeong
request Request a Protocol
ask Ask a question
Favorite

The mechanical properties of a DNA film were characterised using nanoindentation (NanoIndenter XP; MTS, Eden Prairie, MN, USA) with a Berkovich diamond indenter. The Oliver–Pharr method was utilized to analyse the nanoindentation data to determine the representative modulus values with respect to the indentation depth55. The surface stiffness of the DNA film was measured based on the continuous stiffness measurement unit obtained using the NanoIndenter XP, and Poison’s ratio of DNA was assumed to be 0.3. The measured modulus was 5.39 GPa, which reflected the average value over a range of indentation depths from 100 to 200 nm. The tests were repeated in nine trials, using the same samples.

Copyright and License information: The Author(s) ©2017
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A