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Bronchoalveolar Lavage Fluid

EB Ella Bhagyaraj
HW Hongbin Wang
XY Xinghong Yang
CH Carol Hoffman
AA Ali Akgul
ZG Zakia I. Goodwin
DP David W. Pascual
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Nasally infected and uninfected mice were euthanized, their tracheas were cannulated, and the lungs were perfused with 1 ml of cold sPBS three times, and a total of 3 ml BAL fluids were collected from each mouse. BAL fluid samples were centrifuged at 500 × g for 10 min at 4°C, and recovered fluids were filter-sterilized using 0.22 µ syringe filters. The sterile BAL fluid samples were stored at −70°C for cytokine analysis.

Copyright and License information:  ©2021 Bhagyaraj, Wang, Yang, Hoffman, Akgul, Goodwin and Pascual
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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