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4.5. In-Vitro Determination of Nitric Oxide (NO) Production
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After 24 h of incubation, nitric oxide (NO) assay was performed in HT-22 and PC-12 cells according to the Griess reaction method [57]. An amount of 100 mL of cell-free supernatant was collected from each well plate, and 100 mL Griess reagent was added. Curcumin (10 µM) was used as a positive control. The standard curve was calculated by using known concentrations of sodium nitrite, and the absorbance was measured at 540 nm using a microplate reader.
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