4.2. MIN6 Cell Culture

The murine insulinoma MIN6 cells were generously donated by Prof. Peter Bergsten (Uppsala University, Uppsala, Sweden) with the permission of Dr. Jun-ichi Miyazaki (Division of Stem Cell Regulation Research, Osaka University, Osaka, Japan) [53]. The culture medium consisted of DMEM containing 4.5 g/L glucose, 10% fetal bovine serum, 50 μM β-mercaptoethanol, 200 mg/L penicillin, 50 mg/L neomycin, 2.5 mg/L amphotericin B. The culture was conducted at 37 °C in a humidified atmosphere of 5% CO₂. The experiments were performed between passages 24–30.