2.11. Microglia phagocytosis assay

Primary microglia were seeded in a 12-well plate glass slides as described [9]. The cells were then pretreated with Qu and stimulated with LPS for 24 h. To assess microglial phagocytotic activity, 0.5 μl/ml latex beads (2-μm, Invitrogen) were added into each well as described [34]. After 2 h, the cells were washed with PBS and fixed with 4% PFA for 10 min at room temperature then rinsed with PBS and observed by fluorescence microscopy (Olympus, Tokyo, Japan).