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2.8. Mitochondria Isolation
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Mitochondria isolation from HepG2 cells was performed by Mitochondrial Isolation kit (Thermo, Rockford, IL, USA) in accordance with manufacturer’s instructions. Cell lysates were homogenized with commercial kit reagents and centrifuged twice for discarding impurities. The mitochondrial pellets were resuspended and the concentration was determined by the BCA protein assay kit.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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