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6-OHDA Toxicity Assay in vitro
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DA neurons were incubated with 200 μM 6-OHDA (Sigma) for 2 h and incubated at 37°C. Medium was removed and fresh medium was added. Twenty-four hours later, cells were fixed by evaluation by immunocytochemistry. This neurotoxin is taken up by the DA Transporter and therefore does not affect other neurons present in the differentiated cultures.
Copyright and License information: ©2021 Lara-Rodarte, Cortés, Soriano, Carmona, Rocha, Estudillo, López-Ornelas and Velasco.
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