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LGG membranes were labeled using PKH26 Fluorescent Cell Linker Kits following the manufacturer's instructions. 1 × 108 CFU LGG or LELN-LGG were incubated with 1 μL PKH26 dye for 1 min at 37°C, followed with 2 washes in ice-cold 1× PBS to remove free dye. Labeled LGG was covered with aluminum foil to protect from light. The percentage of PKH26 positive and PKH26 fluorescent intensity were detected by FACs (BD Canto) according to a previous report (Teng et al., 2018).
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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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