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Trypan blue dye exclusion

SB Shilpa Bhatia
KH Kellen Hirsch
SB Sanjana Bukkapatnam
NB Nimrah A. Baig
AO Ayman Oweida
AG Anastacia Griego
DC Dylan Calame
JS Jaspreet Sharma
AD Andrew Donson
NF Nicholas Foreman
CA Christopher Albanese
SV Sujatha Venkataraman
RV Rajeev Vibhakar
SK Sana D. Karam
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DAOY and UW228 cells were seeded at a density of 150,000 cells per well in six-well plates and maintained in DMEM containing 10% FBS and primocin. Cells were transfected as indicated above and replated at a density of 30,000 cells/well in a six-well plate. Approximately 24 h post-plating, cells were exposed to 8 Gy dose of radiation. After 120–144 h post-radiation, cells were collected by trypsinization. To each 10 μL aliquot of cells, 10 μL of trypan blue dye was added. Cell counting was performed using the T-20 automated cell counter (Bio-Rad, Hercules, CA, USA).

Copyright and License information: The Author(s) ©2017
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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