Magnetic bead isolation of cells

To isolate specific populations of cells, single-cell suspensions as isolated above are preblocked with anti-CD16/32 for 15 min at 4°C. We then used the biotinylated anti-Gr1 (clone RB6-8C5), anti-CD8β, or anti-CD163 antibodies (all from Thermo Fisher Scientific) to label murine myeloid, CD8⁺, and human monocytes, respectively. Next, the cells were washed and then incubated with anti-biotin magnetic beads (Miltenyi Biotec) before performing manual positive selection using MS columns (Miltenyi Biotec). Purified cells were analyzed for all downstream metabolic analyses.