Flow cytometry

LS Lijuan Shao
QH Qian He
JW Jingbo Wang
FH Fei He
SL Shengcheng Lin
LW Liujing Wu
YG Yubiao Gao
WM Wei Ma
JD Jun Dong
XY Xiaofei Yang
FL Furong Li
request Request a Protocol
ask Ask a question
Favorite

For cell preparation, tumor tissues were dissociated by using according to the instructions, and CD8 T cells were purified by magnetic beads (Miltenyi, 130-045-201). For cultured cells, cells were harvested and adjusted to 1 × 105/100 μl, stained with antibodies (PD-L1-PE, BioLegend, 329705; B7-H3-PE, BioLegend, 331605; CD8-FITC, BioLegend, 344704; IFN-γ-APC, BioLegend, 502511; TNF-α-APC, BioLegend, 502913; IL-10-APC, BioLegend, 501409; IL-1β-APC, 17-7114-80, Thermo; Mouse IgG1-PE, BioLegend, 400112; Mouse IgG1-FITC, BioLegend, 400107; Mouse IgG1-APC, BioLegend, 400119) for 30 min, washed once, and centrifuged at 900 r/min. Finally, the supernatant was removed and resuspended in PBS for flow cytometry. The results of flow cytometry were analyzed using FlowJo software.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A