For cell preparation, tumor tissues were dissociated by using according to the instructions, and CD8 T cells were purified by magnetic beads (Miltenyi, 130-045-201). For cultured cells, cells were harvested and adjusted to 1 × 105/100 μl, stained with antibodies (PD-L1-PE, BioLegend, 329705; B7-H3-PE, BioLegend, 331605; CD8-FITC, BioLegend, 344704; IFN-γ-APC, BioLegend, 502511; TNF-α-APC, BioLegend, 502913; IL-10-APC, BioLegend, 501409; IL-1β-APC, 17-7114-80, Thermo; Mouse IgG1-PE, BioLegend, 400112; Mouse IgG1-FITC, BioLegend, 400107; Mouse IgG1-APC, BioLegend, 400119) for 30 min, washed once, and centrifuged at 900 r/min. Finally, the supernatant was removed and resuspended in PBS for flow cytometry. The results of flow cytometry were analyzed using FlowJo software.
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