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Eight-week-old male mice PDSS2 wild type [WT (n = 6)], PDSS2−/−, Nrf2−/−, Nrf2+/+, on C57BL/6 background (18–22 g) were provided by Nanjing Medical University (Nanjing, China). PDSS2−/− mice (n = 12) were crossed with PDSS2−/− to obtain PDSS2−/−, Nrf2−/− (n = 6) and PDSS2−/−, Nrf2+/+ (n = 6) mice. Mice were fed an AIN76A Western diet for 8 weeks to accelerate the development of AS. Mice from each group were euthanized after 8 weeks. The experimental scheme was approved by the Animal Care of the First People's Hospital of Lianyungang.

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