Quantification of vessel density and pericyte coverage

Johanna Schaffenrath; Sheng-Fu Huang; Tania Wyss; Mauro Delorenzi; Annika Keller

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Quantification of vessel density and pericyte coverage

JS Johanna Schaffenrath

SH Sheng-Fu Huang

TW Tania Wyss

MD Mauro Delorenzi

AK Annika Keller

This method is extracted from research article: Fluids Barriers CNS, Jul 2021

Characterization of the blood–brain barrier in genetically diverse laboratory mouse strains

DOI: 10.1186/s12987-021-00269-w

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Sagittal Sections (70 µm thick) were stained with antibodies against collagen IV (COL-IV) and aminopeptidase N (CD13). Images of 22 µm z-size were analyzed using FIJI version 11 [60]. For vessel density, a slightly modified vessel analysis plugin (version 1.1) in FIJI was used, where 3 regions of interest (ROI) were measured in images taken from hippocampus or from cortex. To determine vessel pericyte coverage, images were converted to binary and 6 (150 μm × 150 µm) regions of interest were analyzed in COL-IV and CD13 channel using the area measurement tool. The percentage of signal overlay was calculated to determine the vessel pericyte coverage.

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